Features

· Accurate sample preparation method support
· Analysis method setup with powerful & easy to use Chromatography Data System
· Customized application support with recommended columns and consumables
· Superior cost effectiveness with optimized analysis efficiency
· Low maintenance cost and long term stability

Specification :

In the food industry, the quality management for products is conducted by analyzing esterified fatty acids to 

determine the ratio of saturated and unsaturated fatty acids in fat. 

There are numerous kinds of fatty acid analysis such as fatty acids in edible oils (ex-sesame oils), recently brought 

up social issue- trans fatty acids, omega 3 fatty acids (refined fish oils) in healthy functional food, etc. 

For fatty acids have many isomers to be separated, they are commonly analyzed by gas chromatograph after 

esterified to Fatty Acid Methyl Esters (FAMEs) to avoid peak tailings by a direct injection of fatty acid and column 

clogging. 

It’s very important to set an optimal oven program because the polar or moderately polar capillary columns used in 

this analysis have low temperature limit. 

The recommended column for the analysis of fatty acids in edible oils is polar capillary columns such as Omegawax 

of Innowax, and for trans fatty acids is moderately polar capillary columns such as HP-23 or SP-2560 to separate cis/

trans isomers. The order of peaks must be indentified first for the eluted peaks of fatty acids are different in the use of 

polar or moderately polar capillary columns.

YL Fatty Acid Analyzer is suitable to analyze various fatty acids and provide all the solution from preparation of 

sample to analysis.

(1) Sample

• Lipolysis : 

Sample 25mg  Add 0.5N NaOH methanol solution 1.5 ml and mix

  Heat at 100 °C for 5 min  Cool down to 30-40 °C 

 • Fatty Acids Esterification:

  Add 14 % Trifluoroborane methanol solution 2 ml and mix 

  Heat at 100 °C for 2 min 

  Cool down to 30-40 °C, add 1 ml of hexane (or heptane) and stir it for 30 sec.

  Add 5 ml of saturated NaCl solution and stir 

  Separate layers at the ambient temperature

  Use an upper layer (Hexane or hexane layer) as a sample

(2) Standard 

 Fatty acids in edible oils –14 kinds of Fatty acid methyl ester mix

 Trans fatty acids- 37 kinds of Fatty acid methyl ester mix

 cis, trans isomer standard of 18:2, 18:3 

 Omega 3 fatty acids – DHA, EPA standard

• Application

- Fatty acids in edible oils 

- Trans fatty acids 

- Omega 3 fatty acids (DHA, EPA)