High-Performance Liquid Chromatograph Primaide Hitachi Japan
High-Performance
Liquid Chromatography (HPLC) is utilized in a wide range of fields, such as
medicine, food production, chemistry, and environmental science, making the
demand for HPLC systems higher among the various types of analytical equipment.
The Hitachi Primaide HPLC is designed for long-term, stable operation, and
features both high reliability and superior durability. It offers tremendous
capability for everyday analysis.
Features
- Simple operation for any HPLC
user
Even in small to mid-scale facilities, individual operators see increasing
opportunities to use multiple HPLC systems. Thanks to its simple design
and front-access layout, maintenance of the Primaide is easy. Even a
beginner at LC analysis can confidently operate the Primaide.
- Excellent basic performance
The demand for HPLC systems with higher precision, better reproducibility,
and improved stability is increasing. The Primaide is built according to
strict specifications to meet such stringent requirements. In addition,
labs can apply the Primaide to an even wider range of applications by
employing optional units, such as a sophisticated DAD (Diode Array
Detector) and an autosampler cooling unit.
- Reliable durability
Hitachi HPLC systems have earned a reputation as highly durable machines,
and the Primaide is no exception. Our strict production quality standards
ensure both high performance and endurance. These standards are applied to
all equipment from systems to accessories.
System
Features
- Optional units mounted inside
provide clean appearance
All principal options, including a degasser, gradient unit, and sample
cooling system can be mounted inside the main body. It is not necessary to
make room for optional units.
- Front access layout for improved
operation and maintenance
Every module is accessed and handled from the front. Since connections are
arranged on the front, it is easy to replace columns and manipulate the
flow system. For easier maintenance, the lamp and cell unit are also
easily accessible.
- Leak sensors in all modules
Any leakage in the flow system is detected by leak sensors built into each
module. After detection the system halts, ensuring improved safety and
reliability.
- Complete control with system
manager
A control panel is not necessary, as everything can be controlled via the
system manager. By installing a UI- pad (optional), each module can be
controlled separately.
- e-Line and USB
The e-Line is an advanced version of the existing D-Line. All modules are
connected by an e-Line in a single chain, and all the communications are
integrated. The use of digital signals improves the reliability of data
transactions. The interface between the PC and equipment is via versatile
USB. A wide range of PCs are supported, enabling flexible configuration.
- GLP function
A Hg lamp is installed in the detector, and wavelengths in the UV range
can be calibrated using its emission line (254 nm) (installed in the
Primaide 1410 UV / 1430 DAD). Each module stores various types of quality
assurance information, for improved data reliability.
Module
lineup:
1110
Pump
The
Primaide 1110 Pump has been developed for reliable analysis and shares
characteristics with Hitachi's existing HPLC pump models. Its consistently high
level of performance provides users with strong support in everyday analysis.
- Improvement of flow rate
stability
For pulse compensation, a proprietary real-time feedback method has been
developed for Hitachi’s pumps. Not only has the pulse been significantly
reduced, but the Primaide 1110 Pump estimates pressure fluctuations and
employs the optimal timing to set the plunger speed interval.
- Improvement of retention time
reproducibility
For gradient analysis, injection by the Primaide 1210 Autosampler is
synchronized with the movements of the pump. This ensures a highly
reproducible retention time, and precise analysis.
Gradient Performance Evaluation/RT and Area Reproducibility
|
Peak No.
|
Component
|
Retention Time
|
Average / min.
|
R.S.D. / %
|
1
|
Acetanilide
|
2.032
|
0.076
|
2
|
Acetophenone
|
2.793
|
0.080
|
3
|
Propiophenone
|
3.581
|
0.096
|
4
|
Butyrophenone
|
4.495
|
0.102
|
5
|
Benzophenone
|
4.809
|
0.098
|
6
|
Valerophenone
|
5.639
|
0.083
|
7
|
Hexanophenone
|
6.975
|
0.082
|
8
|
Heptanophenone
|
8.413
|
0.068
|
9
|
Octanophenone
|
9.882
|
0.078
|
Condition
|
Column
|
HITACHI LaChrom C18 (5 µm) 4.6 mm I.D.×150 mm L.
|
Mobile phase
|
A : CH3OH B : H2O
|
Gradient program
|
0 - 11 min A 65 → 100%
|
Column Temperature
|
40°C
|
Flow rate
|
1.0 mL/min
|
Detection
|
UV 265 nm
|
Injection vol.
|
10 µL (100 g/mL each.)
|
|
|
|
|
|
1210
Autosampler
The
Primaide 1210 Autosampler incorporates direct injection, allowing the
automation of consecutive analyses without wasting precious sample.
- Direct injection of
small-quantity samples and quicker analysis
In the direct injection method, the sampling needle becomes part of the
flow channel so that the sample in the needle flows into the channel with
zero waste. This is a great advantage in the analysis of valuable
small-quantity samples. In addition, since the injection method is simpler
than using a sampling loop, the injection cycle is shortened by half. This
method accelerates throughput and improves efficiency in the analysis of
many samples.
- Improved reproducibility results
in high-precision analysis
The syringe for sample measurement is driven by a high-performance motor.
The drive improves the reproducibility of injection. Furthermore, peak
retention times are also more repeatable due to synchronization of
injection with the pump motion, achieving higher analytical reliability.
Injection
Volume Reproducibility
Condition
|
Sample
|
Methyl paraben
|
Mobile phase
|
CH3OH : H2O = 60 : 40
|
Flow rate
|
1.0 mL/min
|
Detection
|
UV 265 nm
|
1310
Column Oven
The
Primaide 1310 Column Oven also functions as a cooler. The preheating function
obtains better performance from columns, achieving sharper peak shapes.
- More resolved peaks with
optimized heating
Pre-heating with an efficient block heater improves the symmetry and
sharpness of peaks, eliminating the influence of ambient temperature
changes. The column installation space, which features an air circulation
system, permits the easy mounting and detaching of columns.
- Cooling function is included as
standard
The oven can regulate the temperature from 5°C to 65°C.
1410
UV Detector
1430
Diode Array Detector
For More Details: 01733 229386